鑫达医学翻译公司-最好的医学翻译、病历翻译、药学翻译、sci论文翻译、生物翻译网站!您最好的医学论文翻译合作伙伴!
设为首页 | 加入收藏 | 联系我们
首页 > 翻译讲堂 >

经典SCI写作句型总结

发布者:鑫达医学翻译 发布时间:2014-04-01阅读:

写Western blot,怕遗漏重点?八步搞定!
 
1. 扣个帽子(借用文献来简化写作)
Western blotting analysis was performed as previously described (reference). 
 
2. 获取蛋白
The brain tissues were homogenized in RIPA lysis buffer containing protease and phosphatase inhibitors. 
 
3. 跑SDS-PAGE胶,转膜
Proteins were separated by electrophoresis on a 10% SDS-PAGE gel and transferred to a Hybond-P PVDF membrane. 
 
4. 添加一抗
The membrane was blocked in 5% nonfat milk and incubated with an PAR-1 primary antibody (1:1000 dilution,厂家及地址) overnight at 4 °C.
 
5. 添加二抗
Then incubated with the peroxidase-conjugated rabbit anti-goat secondary antibody (1:1000 dilution厂家及地址) for 1 h at room temperature. 
 
6. 显色
After washing with PBS, the bound primary antibody was visualized with the Enhanced Chemiluminescence System from Amersham (Piscataway, NJ, USA) and exposed to film.
 
7. 设置内参
The same membrane was probed for β-actin for loading control. 
 
8. 结果评价
The relative density of PAR to β-actin was analyzed with the TotalLab TL120 Image software (设备型号,厂家及地址).
 
qRT-PCR有固定的写作模式,稍作变动即可!
 
1.RNA提取
 
Total RNA was extracted/isolated from (某组织或器官) using XX Kit (试剂盒名称、厂家及地址) according to manufacturer's instructions.
 
2.cDNA合成
 
The cDNA synthesis was carried out/performed/conducted with approximately 2 µg RNA using XX Kit (试剂盒名称、厂家及地址) with XX enzyme (合成酶的名称、厂家及地址).
 
3.扩增所用设备及引物
 
qRT-PCR was carried out/performed/conducted using 染料 (染料名称、厂家及地址) on a(n) XX system (设备型号、厂家及地址) with the primers listed in table 1.
 
4.定量
 
The mRNA level was normalized by beta-actin.
 
5.PCR扩增体系
 
PCRreactions were performed in a total volume of 10 µl containing 5 µl 2×SYBRPremix, 0.2 µl each specific primer to a final concentration of 200 nM, and 1µl cDNA template.
 
6.PCR扩增条件
 
The PCR conditions consisted of denaturation at 95°C for 3 min, followed by 35cycles of denaturation at 95°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 30 s.
 
7.结果分析
 
The amplification results for real-time PCR was calculated as 2(-ΔΔCt), according to the previous description (reference).
 


鑫达医学翻译公司本着“专业、专注、诚信、共赢”的原则,旨在为广大客户提供生物学、医学、药学材料的口译与笔译,业务范围为医学英语翻译病历翻译医学文献翻译药学翻译SCI医学论文翻译以及写作与投稿,宣传册制作,项目申报材料及医疗类中英文网站的建设工作。
联系方式

免费咨询电话:400-6870-112
传真:0531-88956115
地址:山东省济南市高新区舜华路2000号舜泰广场6号楼27层CD区
邮箱: trans@xdtrans.com