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分子生物学SCI论文翻译

发布者:鑫达医学翻译公司 发布时间:2011-06-24 8:50:52 阅读:

原文
核酸提取
根据香草醛和DMACA染色结果发现,在授粉后15d左右,种皮中有原花色素前体物质如表-3-羟基黄烷酮类和3-羟基花色素类等物质,授粉后约25d表3-羟基黄酮类合成达到高峰,按照文献方法提取授粉后15 d和25 d种皮的总RNA。采用CTAB法提取叶片总DNA。
芥菜型油菜 TT1全长cDNA克隆和DNA序列的克隆
用DNase I (TaKaRa) 消化RNA 样品中的DNA 后, 按TOYOBO 公司的反转录酶操作说明将其反转录成cDNA 后作为PCR 模板。依据拟南芥TT1基因的序列设计同源简并引物TT1(序列见表1),以紫叶芥授粉后15 d种皮的RNA反转录的产物为模板,扩增TT1基因cDNA部分序列。

译文
Nucleic Acid Extraction
Precursors of proanthocyanidin, such as epi-flavan-3-ols and 3’-hydroxyanthocyan, was detected in seed coat at about 15 DAP according to the dyeing results of vanillina and DMACA. The synthesis of epi-flavan-3-ols reached the peak level at about 25 DAP. RNA from seed coat at 15 and 25 DAP was extracted according to the previous report. Total DNA was extracted from the leaves according to the CTAB method.
Cloning of TT1 full-length cDNA and DNA sequences in Brassica juncea
DNase I (TaKaRa) was used to digest the DNA in the RNA samples. cDNA was obtained by the reverse transcription protocol (TOYOBO), and was used as the template for PCR amplifications. Homology-degenerate primers TT1 were designed based on the TT1 gene sequence in A. thaliana (Table 1). The reverse transcription product of RNA extracted from purple-leaf mustard seed coat at 15DAP was used as the template to amplify part of TT1 cDNA sequence.


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