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发布者:鑫达医学翻译公司 发布时间:2011-06-26 8:50:52 阅读:

原文
等位基因特异PCR检测TT1基因SNP的结果验证了黑籽近等基因系NILB的序列和轮回亲本四川黄籽序列一致,黑籽近等基因系NILA的序列和供体亲本紫叶芥序列一致,说明只要设计好合适的引物,优化好PCR条件,特异PCR检测基因的SNP是可行的,因此可以利用特异PCR很方便地检测基因的多态性和等位基因的来源。在转基因芸薹属的基因漂移分析中, 尤其是在缺少转基因筛选标记的情况下,可以利用TT1 基因作为转基因油菜或者芸薹属花粉漂移检测时的标记基因, 可为基因漂移风险分析提供新的方法。

译文
SNP of TT1 gene detected in allele-specific PCR confirmed TT1 sequence of black-seeded rapeseed NILB was consistent with that of recurrent parent Sichuan Yellow, TT1 sequence of black-seeded rapeseed NILA was consistent with that of donor parent purple-leaf mustard. This demonstrated gene SNP could be detected by specific PCR amplifications provided that the suitable primers were designed and the condition was optimized. Therefore, allele-specific PCR could be used to detect gene polymorphism and the source of allele. TT1 gene could be considered as a marker gene for the drift detection in pollen of transgene rapeseed or Brassica, especially under the conditions where the transgene selection markers were not available. This will provide a new method for the gene drift risk analysis.
 

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