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发布者:鑫达医学翻译 发布时间:2013-03-24 8:50:52 阅读:

原文
With increasing knowledge of extraintestinal E.coli pathogenesis in human, the understanding of the relationship among VFs, their antimicrobial resistance and/or susceptibility and their phylogenetic group background has become one focused research point. Recently, some studies have described these relationships [69, 70, 71]. Although previous studies have reported the distribution of extraintestinal E. coli isolates from different VFs based on their specific antimicrobial resistances, the relationship between thirty known extraintestinal E. coli VFs and three drugs (gentamicin, cefepime and piperacillin / tazobactam) have not been reported.

UV light, Used to check the electrophoresis DNA band results; - Gel Documentation and Image Analysis System, ChampGel 3200, machine that was used to analyze the electrophoresis DNA band results; -Pipettes, used to transport and/or measure a specific volume of liquid; -Glassware; -Alcohol lamp, was used for heating the wire loop to prevent contamination to the culture during the inoculations of E. coli bacteria cultures; -Inoculating loop used to retrieve an inoculum from a culture of E. coli; -Tubes: Centrifuge tubes, PCR tubes etc; -Reagents: Gram stain reagents, Agarose gels, Tryptone,Yest, NaCl, 2-MPA, EDTA, LB medium, Distilled water, PCR reagents, TBE buffer, DNA marker, Ethidium bromide, Chloroform,Glycerol, Normal saline.

译文
随着对肠外致病性大肠杆菌在人体发病机理的深入了解,毒力因子、药物抗性或敏感性及遗传谱系间的关系成为研究的热点。近期发表的相关文献对其关系进行了报道。虽然既往发表文献对肠外致病性大肠杆菌的毒力因子分布进行了报道,但30种已知肠外大肠杆菌毒力因子和3种药物(庆大霉素,头孢吡肟和哌拉西林/三唑巴坦)之间的关系尚未明确。

紫外光:查看电泳后DNA片段大小。凝胶成像系统(ChampGel 3200):分析电泳后DNA条带结果。移液枪:液体转移。玻璃器皿、酒精灯:用于接种环加热、灭菌,防止培养过程中出现感染。接种环:自大肠杆菌培养基内获取接种细菌。管:离心管及PCR管等。试剂:革兰染色剂、琼脂糖凝胶、胰蛋白胨、酵母、NaCI、2-MPA、EDTA、LB培养基、蒸馏水、PCR试剂、TBE buffer、DNA marker、溴化乙锭(EB)、氯仿、甘油及生理盐水。


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