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发布者:鑫达医学翻译 发布时间:2013-02-14 8:50:52 阅读:

原文
吸收系数 取供试品约20mg,精密称定至20ml量瓶中,加水溶解并稀释成每1ml约含1.0mg的溶液,摇匀,精密量取2ml至100ml量瓶中,用碳酸氢钠溶液(42g→1000ml)稀释至刻度,照紫外-可见分光光度法检验操作规程检验,在262nm波长处测定吸收度,吸收系数为190~210。
鉴别
(1)在含量测定项下记录的图谱中,供试品溶液主峰的保留时间应与头孢西丁对照品溶液主峰的保留时间一致。
(2)本品的红外光吸收图谱应与对照的图谱一致。
(3)本品显钠盐的火焰反应。
酸度 取供试品约1g,加新煮沸放冷的纯化水10ml,制成每1ml中约含0.1 g的溶液,照pH值检验操作规程依法测定,pH值应为4.2~7.0。

译文
Absorption coefficient
Transfer 20 mg of test samples, accurately weighed, to a 20 ml volumetric flask. Then the sample was diluted with purified water to a final concentration of 1.0 mg/ml, followed by gentle agitation. Subsequently, 2 ml test solution was accurately transferred to a 100 ml volumetric flask, and diluted using 0.5 mM sodium bicarbonate. Ultraviolet/visible spectrophotometry was performed to test the standard operation procedure (SOP). A wavelength of 262 nm was chosen. The AC of the test sample was in a range of 190 ~ 210.
Identification
(1) The retention time of the major peak in the chromatogram for the test solution was consistent with that of the cefoxitin (control). The retention time of the major peak for tylosin A in the chromatogram of theTest solutioncorresponds to that in the chromatogram of theStandard solution, as obtained in the test forContent of tylosins.
(2) The infrared absorption spectroscopy in the chromatogram of the test solution corresponded to that in the chromatogram of the standard solution. 
(3) Flame test detected the presence of sodium salt. 
Acidity
To evaluate the acidity of the test sample, 1 g sample was dissolved using 10 ml cooled boiling purified water. The acidity of the solution was determined using a PH meter. The pH value of the solution ranged from 4.2 to 7.0.


 


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